Anti-RPT2a | Regulatory particle triple-A ATPase subunit 2a

RPT2a (Regulatory particle triple-A ATPase subunit 2a) is the 26S proteasome subunit. It is required for root meristem maintenance, and regulates gametogenesis. RPT2a is also shown to regulate gene silencing via DNA methylation. Alternative names: 26S proteasome regulatory subunit 4 homolog A Alternative name (s) : 26S proteasome AAA-ATPase subunit, RPT2a 26S proteasome subunit 4 homolog A, Protein HALTED ROOT, 26S proteasome regulatory subunit 4 homolog A.

Recombinant, full-lenght RPT2a of Arabidopsis thaliana RPT2a protein sequence UniProt: Q9SZD4-1, TAIR: At4g29040 overexpressed in E.coli, purified from a gel piece

This antibody is also recognizing RPT2b protein.Recommended western blot conditions:SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.For an image of western blot detection, refer to:Smalleet al. (2002).

Pang et al. (2025) . The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis. Nat Commun. 2025 Feb 27;16 (1) :2039. doi: 10.1038/s41467-025-57306-7. Meng, Wang, Hao, et al. (2023) RNA-binding protein MAC5A interacts with the 26S proteasome to regulate DNA damage response in Arabidopsis. Plant Physiol. 2023;191 (1) :446-462. doi:10.1093/plphys/kiac511Smalle et al. (2002) . Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

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