Anti-PAC1 | 20S Proteasome alpha subunit C1
Product Specifications
Background
PAC1 (20S Proteasome alpha subunit C1)is a component of proteasome complex which has ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The process is ATP-dependent.Alternative names: Proteasome subunit alpha type-4-A, 20S proteasome alpha subunit C-1, Proteasome 27 kDa subunit, Proteasome component 9, Proteasome subunit alpha type-3
Host
Rabbit
Reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Recombinant PAC1 ofArabidopsis thaliana,UniProt:O81148-1, TAIR:At3g22110, overexpressed inE.coli, purified from a gel piece
Clonality
Polyclonal
Applications
Western blot (WB)
Dilution
1 : 3000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Molecular Weight
27.4 | 26 kDa
Precautions
Recommended western blot conditions:SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.For an image of western blot detection, refer to:Smalleet al. (2002).
References & Citations
Xieet al. (2024). Proteasome resides in and dismantles plant heat stress granules constitutively. Mol Cell. 2024 Sep 5;84(17):3320-3335.e7. doi: 10.1016/j.molcel.2024.07.033.Smalleet al. (2002). Cytokinin growth responses in Arabidopsis involve the 26S proteasome subunit RPN12. Plant Cell 14, 17-32.
Storage Conditions
Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc670241/datasheet/as19 4258.pdf
CAS Number
9007-83-4
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