Anti-GFP | Green fluorescent protein (VENUS)
Product Specifications
Background
Green fluorescent protein (Venus) from Aequorea victoria (Jellyfish), can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when THR-203 is replaced by Tyr) . It is an energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer.
CAS Number
9007-83-4
HS Code
38221900
UniProt
P42212
Host
Rabbit
Reactivity
Cell lysate overexoressing Venus protein fusion
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Full length, recombinat VENUS protein, expressed in E.coli, UniProt: P42212
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
BFP/FITC/GFP/RFP/TRITC/YFP
Dilution
1 : 1000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Molecular Weight
26 kDa
References & Citations
Zhang et al. (2024) . An epigenetically mediated double negative cascade from EFD to HB21 regulates anther development. Nat Commun. 2024 Sep 6;15 (1) :7796. doi: 10.1038/s41467-024-52114-x. Baiden et al. (2022) . Heterologous expression of antimicrobial peptides S-thanatin and bovine lactoferricin in the marine diatom Phaeodactylum tricornutum enhances native antimicrobial activity against Gram-negative bacteria, Algal Research, Volume 69, 2023, 102927, ISSN 2211-9264, https://doi.org/10.1016/j.algal.2022.102927.
Storage Conditions
Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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