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Anti-Pectic polysaccharide, beta-1,4-galactan (monoclonal, clone LM5)

Product Specifications

Background

The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Homogalacturonan is a pectic polysaccharide of alpha-1,4 linked galacturonic acid residues. Pectin contains a complex set of polysaccharides that can be found in many primary cell walls.

CAS Number

9007-83-4

HS Code

38221900

Host

Rat

Reactivity

Higher plants, ferns and mosses

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

Pectic polysaccharide (beta 1,4-galactan)

Clonality

Monoclonal

Applications

ELISA (ELISA), Immunoflourescence (IF)

Field of Research

Dr. Knox collection, University of Leeds

Dilution

1:10 (ELISA, IF)

Purity

Cell culture supernatant.

Format

Liquid

Additionnal Information

Contains 0.05% Sodium Azide.No cross-reactivity with (1-3) -beta-D-galactans or (1-6) -beta-D-galactans.It recognizes a linear tetrasaccharide in (1-4) -beta-D-galactans.In ELISA (competitive inhibition), antibody is binding to: (1-4) -beta-D-galactan was inhibited (50%) by 58 µg/ml (1-4) -beta-D-galactotetraose and by 0.7 µg/ml lupin (1-4) -beta-D-galactan.

References & Citations

Jacquer et al. (2025) . A developmental switch controls cell-to-cell transport in roots via pectin-linked plasmodesmata changes. Mol Plant . 2025 Jul 17:S1674-2052 (25) 00233-3. doi: 10.1016/j.molp.2025.07.004. Albornos et al. (2025) . Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage.Planta. 2025 May 24;262 (1) :4. doi: 10.1007/s00425-025-04717-x.Andersen et al. (2016) . Characterization of the LM5 pectic galactan epitope with synthetic analogues of ß-1,4-d-galactotetraose. Carbohydr Res. 2016 Dec ;436:36-40.doi: 10.1016/j.carres.2016.10.012. Jones et al. (1997) . Development and validation of an in vitro model system to study peripheral sensory neuron development and injury. Sci Rep. 2018 Oct 29;8 (1) :15961. doi: 10.1038/s41598-018-34280-3.

Storage Conditions

Store at +4°C (short term) and at -20°C (long term) . Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube.

Isotype

IgG

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