Anti-mCherry (chicken antibody)

Product Specifications

Background

MCherry is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, however it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer) . The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals) .

CAS Number

9007-83-4

HS Code

38221900

Host

Chicken

Reactivity

MCherry

Immunogen

Recombinant full length mCherry expressed and purified from E. coli.

Clonality

Polyclonal

Applications

Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)

Field of Research

BFP/FITC/GFP/RFP/TRITC/YFP

Dilution

1 : 1000 (ICC), 1: 2000 (IF), 1 : 2000-1 : 5000 (WB)

Purity

Purified, total IgY (chicken egg yolk immunoglobulin) with 3% trehalose.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Precautions

In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.