Anti-mCherry (chicken antibody)
Product Specifications
Background
mCherryis derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is a 223 amino acid ~28kDa protein similar in size and properties to GFP, however it produces a red rather than a green fluorochrome. mCherry in its monomeric form is useful for applications such as Förster Resonance Energy Transfer (FRET, also known as Fluorescence Resonance Energy Transfer). The protein is an engineered derivative of one of a family of proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals).
Host
Chicken
Reactivity
mCherry
Immunogen
Recombinant full length mCherry expressed and purified fromE. coli.
Clonality
Polyclonal
Applications
Immunocytochemistry (ICC), Immunofluorescence (IF), Western blot (WB)
Dilution
1 : 1000 (ICC), 1: 2000 (IF), 1 : 2000-1 : 5000 (WB)
Purity
Purified, total IgY (chicken egg yolk immunoglobulin) with 3% trehalose.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Precautions
In western blot ~28 kDa band is detected corresponding to intact full-length mCherry on HEK293 cells transfected with mCherry vector
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc670353/datasheet/as18 4178.pdf
CAS Number
9007-83-4
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