Anti-Phosphothreonine

Product Specifications

Background

Phosphothreonineis a phosphoamino acid and phosphorylated ester of threonine.

Host

Rabbit

Reactivity

Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.!!AIR8!!Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated phosphothreonine

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)

Dilution

ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)

Purity

Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.

Format

Liquid

Precautions

2 µg/mL of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.

References & Citations

Weise Havshøiet al. (2024). The mechanism behind tenuazonic acid-mediated inhibition of plant plasma membrane H+-ATPase and plant growth. JBC 107167.

Storage Conditions

Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.