Anti-V-ATPase holoenzyme
Product Specifications
Background
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
Avena sativa
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Vacuolar membrane
Dilution
1 : 400 - 1: 1000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
Molecular Weight
100, 44 , 42, 36 , 16, 13 kDa
Precautions
Antibody reacts with V-ATPase subunits VHAa (100 kDa), A, B, 44 , 42, 36 , 16, 13 kDa, and with Calnexin
References & Citations
Ward et al (1992) .Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots. Plant Physiol. 99 (1) :161-9. doi: 10.1104/pp.99.1.161.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Higher plants
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