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Anti-V-ATPase holoenzyme

Product Specifications

Background

Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Avena sativa

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

V-ATPase complex from Avena sativa purified by gel filtration Ward and Sze 1992

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Vacuolar membrane

Dilution

1 : 400 - 1: 1000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Molecular Weight

100, 44 , 42, 36 , 16, 13 kDa

Precautions

Antibody reacts with V-ATPase subunits VHAa (100 kDa), A, B, 44 , 42, 36 , 16, 13 kDa, and with Calnexin

References & Citations

Ward et al (1992) .Dissociation and Reassembly of the Vacuolar H-ATPase Complex from Oat Roots. Plant Physiol. 99 (1) :161-9. doi: 10.1104/pp.99.1.161.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Higher plants

Frequently Asked Questions

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