Anti-GST-tag (rabbit antibody, polyclonal)
Product Specifications
Background
GST-tag (glutathione S-transferase) is a tag added to a protein of interest as a fusion protein to unable purification and detection. The tag is 220 amino acid long, ca. 26 kDa which makes it quite large compare to Myc or FLAG tags.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
GST protein
Clonality
Polyclonal
Applications
ELISA (ELISA), Western blot (WB)
Field of Research
BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...
Dilution
1: 1: 1000 (ELISA), 1: 2000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
26 kDa
Precautions
Overnight incubation of this antibody with dilution 1:5 000 with 0,5 µg of GST-tagged protein produced strong background but works fine with samples of totalEscherichia colilysate if GST-tagged protein is expressed in low amounts.
Additionnal Information
Exact working dilution for each application needs to be determined experimentally
Storage Conditions
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Native and denatured forms of purified GST or GST fusion proteins
Documents
Frequently Asked Questions
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