Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody)
Product Specifications
Background
PIF4 (PHYTOCHROME INTERACTING FACTOR 4)is a transcription factor involved in the phytochrome B signaling pathway. Interacts with APRR1/TOC1 and PIF3 and binds to EGL2 and RGA. Alternative names: AtPIF4, Basic helix-loop-helix protein 9, AtbHLH9, bHLH 9, SRL2, Short under red-light 2, EN102, Transcription factor EN 102, bHLH transcription factor bHLH009.
Host
Goat
Reactivity
Arabidopsis thaliana
Not reactive in
Solanum lycopersicum, Vitis vinifera
Immunogen
KLH-conjugated peptide derived fromArabidopsis thalianaPIF4, UniProt:Q8W2F3, TAIR:AT2G43010
Clonality
Polyclonal
Applications
Western blot (WB)
Dilution
1 : 1000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
48.3 | 60 kDa
Precautions
Material used need to be up to 8 days old as detection in older rosette leaf may fail
References & Citations
Bianet al. (2024). The BBX7/8‐CCA1/LHY transcription factor cascade promotes shade avoidance by activating PIF4. New Phytol . 2024 Nov 8. doi: 10.1111/nph.20256.Fanget al. (2022) TANDEM ZINC-FINGER/PLUS3 regulates phytochrome B abundance and signaling to fine-tune hypocotyl growth. Plant Cell. 2022;34(11):4213-4231. doi:10.1093/plcell/koac236Bajracharya,Xi, Grace, et al. (2022) PHYTOCHROME-INTERACTING FACTOR 4/HEMERA-mediated thermosensory growth requires the Mediator subunit MED14. Plant Physiol. 2022;190(4):2706-2721. doi:10.1093/plphys/kiac412Agrawalet al. (2022) MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis. Plant Physiol. 2022 Aug 1;189(4):2259-2280. doi: 10.1093/plphys/kiac220. PMID: 35567489.Leeat al. (2021) Spatial regulation of thermomorphogenesis by HY5 and PIF4 in Arabidopsis. Nat Commun. 2021 Jun 16;12(1):3656. doi: 10.1038/s41467-021-24018-7. PMID: 34135347; PMCID: PMC8209091.Lee, Paik & Huq. (2020). SPAs promote thermomorphogenesis by regulating the phyB-PIF4 module in Arabidopsis. Development. 2020 Oct 8;147(19):dev189233. doi: 10.1242/dev.189233. PMID: 32994167; PMCID: PMC7561471.Sunet al. (2019). SHB1 and CCA1 interaction desensitizes light responses and enhances thermomorphogenesis. Nat Commun. 2019 Jul 15;10(1):3110. doi: 10.1038/s41467-019-11071-6.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc66fdc1/datasheet/as16 3955.pdf
CAS Number
9007-83-4
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