What sizes are available for Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody)?

Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody) is available in the following size(s): 50 µg.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody)

Q: What is the catalog number for Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody)?

The catalog number for Anti-PIF4 | Phytochrome interacting factor 4 (goat antibody) is AS16 3955.

Product Specifications

Background

PIF4 (PHYTOCHROME INTERACTING FACTOR 4) is a transcription factor involved in the phytochrome B signaling pathway. Interacts with APRR1/TOC1 and PIF3 and binds to EGL2 and RGA. Alternative names: AtPIF4, Basic helix-loop-helix protein 9, AtbHLH9, bHLH 9, SRL2, Short under red-light 2, EN102, Transcription factor EN 102, bHLH transcription factor bHLH009.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

818903

UniProt

Q8W2F3

Host

Goat

Reactivity

Arabidopsis thaliana

Not reactive in

Solanum lycopersicum, Vitis vinifera

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana PIF4, UniProt:Q8W2F3, TAIR:AT2G43010

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Transcription regulation

Dilution

1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

48.3 | 60 kDa

Precautions

Material used need to be up to 8 days old as detection in older rosette leaf may fail

Additionnal Information

PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light) . Extraction of PIF proteins is described in Shen et al. (2007) .

References & Citations

Malakar et al. (2025) . BBX24/BBX25 antagonizes the function of thermosensor ELF3 to promote PIF4-mediated thermomorphogenesis in Arabidopsis. Plant Commun. 2025 May 28:101391.doi: 10.1016/j.xplc.2025.101391. Bian et al. (2024) . The BBX7/8‐CCA1/LHY transcription factor cascade promotes shade avoidance by activating PIF4. New Phytol . 2024 Nov 8. doi: 10.1111/nph.20256. Fang et al. (2022) TANDEM ZINC-FINGER/PLUS3 regulates phytochrome B abundance and signaling to fine-tune hypocotyl growth. Plant Cell. 2022;34 (11) :4213-4231. doi:10.1093/plcell/koac236Bajracharya, Xi, Grace, et al. (2022) PHYTOCHROME-INTERACTING FACTOR 4/HEMERA-mediated thermosensory growth requires the Mediator subunit MED14. Plant Physiol. 2022;190 (4) :2706-2721. doi:10.1093/plphys/kiac412Agrawal et al. (2022) MEDIATOR SUBUNIT17 integrates jasmonate and auxin signaling pathways to regulate thermomorphogenesis. Plant Physiol. 2022 Aug 1;189 (4) :2259-2280. doi: 10.1093/plphys/kiac220. PMID: 35567489.Lee at al. (2021) Spatial regulation of thermomorphogenesis by HY5 and PIF4 in Arabidopsis. Nat Commun. 2021 Jun 16;12 (1) :3656. doi: 10.1038/s41467-021-24018-7. PMID: 34135347; PMCID: PMC8209091.Lee, Paik & Huq. (2020) . SPAs promote thermomorphogenesis by regulating the phyB-PIF4 module in Arabidopsis. Development. 2020 Oct 8;147 (19) :dev189233. doi: 10.1242/dev.189233. PMID: 32994167; PMCID: PMC7561471.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.