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Anti-WHIRLY1

Product Specifications

Background

WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165) . WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804) . In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481) . In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489) . WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673) .

CAS Number

9007-83-4

HS Code

38221900

UniProt

UniProt

Host

Goat

Reactivity

Hordeum vulgare

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated peptide derived from HvWHIRLY1 protein sequence, NCBI

Clonality

Polyclonal

Applications

Immunogold (IG), Western blot (WB)

Field of Research

Transcription regulation

Dilution

1: 200 (IG), 1: 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

References & Citations

Krupinska et al. (2025) . Iron allocation to chloroplast proteins depends on the DNA-binding protein WHIRLY1. Planta. 2025 Jun 17;262 (2) :32. doi: 10.1007/s00425-025-04736-8. Karpinska et al. (2022) . WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles. Biochem J. 2022 Mar 18;479 (5) :641-659. doi: 10.1042/BCJ20210810. PMID: 35212355; PMCID: PMC9022988.

Storage Conditions

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Brachypodium sylvaticum, Oryza sativa, Panicum miliaceum, Setaria italica, Triticum urartuSpecies of your interest not listed? Contact us

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