Anti-WHIRLY1
Product Specifications
Background
WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165) . WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804) . In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481) . In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489) . WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673) .
CAS Number
9007-83-4
HS Code
38221900
UniProt
UniProt
Host
Goat
Reactivity
Hordeum vulgare
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
KLH-conjugated peptide derived from HvWHIRLY1 protein sequence, NCBI
Clonality
Polyclonal
Applications
Immunogold (IG), Western blot (WB)
Field of Research
Transcription regulation
Dilution
1: 200 (IG), 1: 1000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl, of sterile water
References & Citations
Krupinska et al. (2025) . Iron allocation to chloroplast proteins depends on the DNA-binding protein WHIRLY1. Planta. 2025 Jun 17;262 (2) :32. doi: 10.1007/s00425-025-04736-8. Karpinska et al. (2022) . WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles. Biochem J. 2022 Mar 18;479 (5) :641-659. doi: 10.1042/BCJ20210810. PMID: 35212355; PMCID: PMC9022988.
Storage Conditions
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Brachypodium sylvaticum, Oryza sativa, Panicum miliaceum, Setaria italica, Triticum urartuSpecies of your interest not listed? Contact us
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