DISCONTINUED Anti-PTOX | Plastid terminal oxidase
Product Specifications
Background
PTOX (plastid terminal oxidase)is a component of electron transfer chain responsible for desaturation of phytoene, which prevents the generation of reactive oxygen species. It is involved in the differentiation of plastids: chloroplasts, amyloplasts, and etioplasts. PTOX is expressed ubiquitously in plant tissues and is located in the lumen.Synonymes: IM, IM1, immutants, AOX4, alternative oxidase 4, ubiquinol oxidase 4, chloroplastic/chromoplastic
Host
Rabbit
Reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Nicotiana tabacum, Solanum lycopersicum, Spinacia oleracea
Not reactive in
Galdieria sulphuraria, Phaeodactylum tricornutum
Immunogen
Part ofArabidopsis thalianarecombinant plastid terminal oxidase, TAIR:At4g22260, UniProt:Q56X52
Clonality
Polyclonal
Applications
Western blot (WB)
Dilution
1 : 4000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Molecular Weight
30 | 37-41 kDa (Arabidopsis thaliana)
Precautions
In most plants it is a minor polypeptide and consequently enrichment by analyzing membrane fractions for example is recommended
References & Citations
Urban,Rogowski & Romanowska (2022), Crucial role of the PTOX and CET pathways in optimizing ATP synthesis in mesophyll chloroplasts of C3 and C4 plants, Environmental and Experimental Botany, Volume 202, October 2022, 105024, https://doi.org/10.1016/j.envexpbot.2022.105024Pralonet al. (2020). Mutation of the Atypical Kinase ABC1K3 Partially Rescues the PROTON GRADIENT REGULATION 6 Phenotype in Arabidopsis thaliana. Front. Plant Sci., 25 March 2020Bolteet al. (2020). Dynamics of the localization of the plastid terminal oxidase PTOX inside the chloroplast. J Exp Bot. 2020 Feb 15. pii: eraa074. doi: 10.1093/jxb/eraa074.Cournacet al. (2000b). Flexibility in photosynthetic electron transport: a newly identified chloroplast oxidase involved in chlororespiration. Philos Trans R Soc Lond B Biol Sci. 2000 Oct 29;355(1402):1447-54Cournacet al. (2000a). Electron flow between photosystem II and oxygen in chloroplasts of photosystem I-deficient algae is mediated by a quinol oxidase involved in chlororespiration. J Biol Chem. 2000 Jun 9;275(23):17256-62.Josseet al. (2000). A Plastid Terminal Oxidase Associated with Carotenoid Desaturation during Chromoplast Differentiation. Plant Physiol. 2000 Aug;123(4):1427-36.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6700ba/datasheet/as16 3692.pdf
CAS Number
9007-83-4
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