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Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal)

Product Specifications

Background

Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) . Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.

CAS Number

9007-83-4

HS Code

38221900

UniProt

Q03JI6

Host

Rabbit

Reactivity

Staphylococcus aureus

Immunogen

KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6 (peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)

Clonality

Polyclonal

Applications

Immunolocalization (IL), Western blot (WB)

Field of Research

Cas9/GAL4/HRP

Dilution

1 : 100 (IL), 1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

Depends on the MW of the protein that is the fusion partner.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Staphylococcus pyogenes UniProt: Q99ZW2

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