Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal)
Product Specifications
Background
Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) . Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.
CAS Number
9007-83-4
HS Code
38221900
UniProt
Q03JI6
Host
Rabbit
Reactivity
Staphylococcus aureus
Immunogen
KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6 (peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)
Clonality
Polyclonal
Applications
Immunolocalization (IL), Western blot (WB)
Field of Research
Cas9/GAL4/HRP
Dilution
1 : 100 (IL), 1 : 1000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
Depends on the MW of the protein that is the fusion partner.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Staphylococcus pyogenes UniProt: Q99ZW2
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