Anti-H3S10p | Histone H3 (p Ser10) (serum)

Product Specifications

Background

Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Phosphorylation of H3S10 is associated with mitosis.

Host

Rabbit

Reactivity

Human

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide

Clonality

Polyclonal

Applications

Chromatin immunoprecipitation (ChIP), Dot Blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Dilution

2 µg/million cells (ChIP), 1 : 20 000 (Dot), 1 : 2000 (IF), 5 µl (IP), 1 : 100 (WB)

Purity

Serum

Format

Liquid

Molecular Weight

15 kDa

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.