Anti-H3S10p | Histone H3 (p Ser10) (affinity purified)
Product Specifications
Background
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Phosphorylation of H3S10 is associated with mitosis.
Host
Rabbit
Reactivity
Human
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
KLH-conjugated synthetic peptide
Clonality
Polyclonal
Applications
Chromatin immunoprecipitation (ChIP), Dot Blot (Dot), ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Dilution
2 µg/million cells (ChIP), 1 : 20 000 (Dot), 1 : 2000 (IF), 1 : 100 (WB)
Purity
Immunogen affinity purified serum.
Format
Liquid
Molecular Weight
15 | 17 kDa
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6702a0/datasheet/as16 3635.pdf
CAS Number
9007-83-4
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