Anti-Non-fucosylated xyloglucan-3 (clone CCRC-M100)
Selected Variant
AS16 3234-1ml
Size: 1 mL
Product Specifications
Background
Xyloglucans are polysaccharides commonly refered to as hemicelluloses found in the primary cell walls of vascular plants. This antibody binds to galactosylated side-chains of non-fucosylated xyloglucan-3, and appears to preferentially bind to the galactosylated side-chain closest to the reducing end of xyloglucan oligosaccharide sub-units (XXXG) .
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
Acer pseudoplatanus, Arabidopsis thaliana, Solanum lycopersicum, Tamarindus indicus
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
BSA-conjugated sycamore ((Acer pseudoplatanus) ) xyloglucan, covalent binding.
Clonality
Monoclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Field of Research
Dr. Hahn collection, University of Georgia
Dilution
Undiluted or at 1 : 10 (ELISA), (IF), (IHC)
Purity
Cell culture supernatant.
Format
Liquid
Precautions
CCRC-M100 binds only to the xyloglucan sub-unit, XXXG, and shows no cross-reactivity with other xyloglucan sub-units tested, CCRC-M100 shows some cross-reactivity with sycamore pectic polysaccharides and linseed mucilage
Additionnal Information
Exact working dilution needs to be determined by end user
References & Citations
Pattathil et al. (2012) . Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.
Storage Conditions
Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
DicotsSpecies of your interest not listed? Contact us
Isotype
IgM
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