Anti-Rhamnogalacturonan-I backbone (clone CCRC-M35)
Product Specifications
Background
Rhamnogalacturonans (RGs) are pectic polysaccharides found in the cell wall, which contain a repeating dissacharides backbone:α-D-GalpA- (1,2) -α-L-Rhap- (1) The antibody was raised against seed mucilage/MeBSA complex and binds to Rha- (1,4) -GalA- (1,2) -Rha- (1,4) -GalA- (1,2) -Rha- (1,4) pectic backbone.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
MeBSA-conjugated Arabidopsis thaliana seed mucilage (Rhamnogalacturonan I), non-covalent complex. Epitope structure for carabohydrate antigen: Rha- (1,4) -GalA- (1,2) -Rha- (1,4) -GalA- (1,2) -Rha- (1,4) .
Clonality
Monoclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Field of Research
Dr. Hahn collection, University of Georgia
Dilution
Undiluted or at 1 : 10 (ELISA), (IHC), (IF)
Purity
Cell culture supernatant.
Format
Liquid
Precautions
CCRC-M35 binds to the backbone of rhamnogalacturonan I and requires at least two unbranched disaccharide repeats for binding, CCRC-M35 does not bind to branched sections of the backbone and is not sensitive to the identity of the sugar at the non-reducing terminus
Additionnal Information
Exact working dilution needs to be determined by end user
References & Citations
Pattathil et al. (2012) . Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6. Patathil et al. (2010) . A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153 (2) :514-25.doi: 10.1104/pp.109.151985.
Storage Conditions
Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
DicotsSpecies of your interest not listed? Contact us
Isotype
IgM
Documents
Frequently Asked Questions
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