Anti-DYKDDDDK (binds to Sigma FLAG®), HRP conjugated (Clone M2)
Product Specifications
Background
Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to HRP, Horseradish Peroxidase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Immunogen
DYKDDDDK (Sigma FLAG®)
Clonality
Monoclonal
Applications
Western blot (WB)
Field of Research
BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...
Dilution
100 ng/ml (ELISA), 1 : 20-1 : 50 (ICC), 1 : 20-1 : 50 (IF), 3 µg (IP), 1 : 10-1 : 50 (WB)
Purity
Protein A purified.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Additionnal Information
Antibody is provided in: 100 mM NaPO4 (pH 7,4), 100 mM NaCl, 1,5% BSA, 50 mM Sucrose, 0,01 % Thimerosal, Affinity purified antibody has over 95% purity based on SDS-PAGE
Storage Conditions
Store freeze-dried poweder at 2-8°C.Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.
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