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Anti-DYKDDDDK (binds to Sigma FLAG®), HRP conjugated (Clone M2)

Selected Variant

AS16 3222

Size: 100 µg

Product Specifications

Background

Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®) and is directly conjugated to HRP, Horseradish Peroxidase. This is of advantage to shorten assay time by no need to use a secondary antibody to DYKDDDDK.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Immunogen

DYKDDDDK (Sigma FLAG®)

Clonality

Monoclonal

Applications

Western blot (WB)

Field of Research

BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...

Dilution

100 ng/ml (ELISA), 1 : 20-1 : 50 (ICC), 1 : 20-1 : 50 (IF), 3 µg (IP), 1 : 10-1 : 50 (WB)

Purity

Protein A purified.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Additionnal Information

Antibody is provided in: 100 mM NaPO4 (pH 7,4), 100 mM NaCl, 1,5% BSA, 50 mM Sucrose, 0,01 % Thimerosal, Affinity purified antibody has over 95% purity based on SDS-PAGE

Storage Conditions

Store freeze-dried poweder at 2-8°C.Rehydrate with 1.0 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily. Shelf life: 1 year from date of receipt. Prepare working dilution prior to use and then discard.

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