Anti-ACT 2,8,11 | Actin 2, 8, 11 (clone mAb13a)
Product Specifications
Background
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
821411
UniProt
Q8LB94 , Q96292
Host
Mouse
Reactivity
Arabidopsis thaliana, Nicotiana tabacum!!AIR8!!Arabidopsis thaliana, Nicotiana tabacum
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
Full-length recombinant Arabidopsis thaliana Actin-2 (ACT2) expressed in E.coli, Uniprot: Q96292
Clonality
Monoclonal
Applications
Immunohistochemistry (IHC), Western blot (WB)
Field of Research
Cytoskeleton
Dilution
1-2 µg/ml (WB)
Purity
IgG1 immunoglobulin purified on Protein G in 0.1 M Sodium Phosphate, pH 7.4, 0.15 M NaCl, 0.05% (w/v) sodium azide.
Format
Liquid
Molecular Weight
45 | 45 kDa
Precautions
Exact working dilution needs to be determined by end user
Additionnal Information
This antibody is a total IgG fraction, purified on a protein G column. It recognizes actin isoforms ACT2, 8, and 11.
References & Citations
Kandasamy, M.K., et al. (2012) . Plant vegetative and animal cytoplasmic actins share functional competence for spatial development with protists. Plant Cell May;24 (5) :2041-57. doi: 10.1105/tpc.111.095281.Kandasamy, M.K., et al. (2001) . One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation. Plant Cell. Jul;13 (7) :1541-54.Kandasamy, M.K., et al. (1999) . The late pollen-specific actins in angiosperms. Plant Journal. Jun;18 (6) :681-9.
Storage Conditions
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Actinidia deliciosa, Brachypodium sylvaticum, Brassica napus, Brassica oleracea, Cucumis sativus, Dionea muscipula, Euphorbia lathyris, Ficus microcarpa, Gentlisea aurea, Gossypium sp., Hevea brasiliensis, Medicago sativa, Medicago truncatula, Oryza sativa subsp. japonica, Oxytropis ochrocephala, Panax notoginseng, Populus trichocarpa, Phaseolus vulgaris, Prunus avium, Striga asiatica, Theobrom cacao, Torenia fournieri, Trifolium pratense, Vaccinium ashei, Ziziphus jujubaSpecies of your interest not listed? Contact us
Isotype
IgG1
Documents
Frequently Asked Questions
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