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Anti-Rhamnogalacturonan-Ia (clone CCRC-M2)

Product Specifications

Background

Rhamnogalacturonans (RGs) are pectic polysaccharides found in the cell wall, which contain a repeating dissacharides backbone:α-D-GalpA- (1,2) -α-L-Rhap- (1)

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

Acer pseudoplatanus, Arabidopsis thaliana, Cyamopsis tetragonoloba

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

MeBSA-conjugated sycamore rhamnogalacturonan-I (non-covalent)

Clonality

Monoclonal

Applications

ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)

Field of Research

Dr. Hahn collection, University of Georgia

Dilution

Undiluted or at 1 : 10 (ELISA), (IF), (IHC)

Purity

Cell culture supernatant.

Format

Liquid

Additionnal Information

Exact working dilution needs to be determined by end user

References & Citations

Pattathil et al. (2012) . Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6. Patathil et al. (2010) . A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153 (2) :514-25.doi: 10.1104/pp.109.151985.

Storage Conditions

Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Rhamnogalacturonan-I from gum karaya, sycamore and Arabidopsis pectic polysaccharides

Isotype

IgM

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