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Goat anti-Dog IgM Fc specific, Biotin conjugated

Product Specifications

Background

Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Reactivity

Dog IgM, Fc part

Not reactive in

This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion

Immunogen

Purified normal IgM isolated from pooled dog serum

Clonality

Polyclonal

Applications

Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)

Field of Research

Anti-Dog

Dilution

1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal

Purity

Protein G purified goat IgG in PBS pH 7.2.

Format

Lyophilized

Reconstitution

For reconstitution, add 1 ml of sterile distilled water, spin down to remove any insoluble particles.

Precautions

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.No preservative added.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be prepared by adding sterile PBS pH 7.2. Working dilutions should be stored at 4°C, not frozen, and preferably used during the same day. If any precipitation occurs, remove it by centrifugation.

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