Goat anti-Dog IgM Fc specific, Biotin conjugated
Product Specifications
Background
Goat anti-Dog IgM Fc, Biotin conjugated is a secondary antibody which binds to dog IgM Fc part in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Goat
Reactivity
Dog IgM, Fc part
Not reactive in
This antibody does not react with IgG, IgG/Fab fragments and IgA or any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Immunogen
Purified normal IgM isolated from pooled dog serum
Clonality
Polyclonal
Applications
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Field of Research
Anti-Dog
Dilution
1 : 500-1: 4000 (ELISA), 1 : 100-1: 250 (ICC), (IHC), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Purity
Protein G purified goat IgG in PBS pH 7.2.
Format
Lyophilized
Reconstitution
For reconstitution, add 1 ml of sterile distilled water, spin down to remove any insoluble particles.
Precautions
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.No preservative added.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be prepared by adding sterile PBS pH 7.2. Working dilutions should be stored at 4°C, not frozen, and preferably used during the same day. If any precipitation occurs, remove it by centrifugation.
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