Goat anti-Dog IgG Fc specific, Biotin conjugated
Product Specifications
Background
Goat anti-Dog IgG Fc, Biotin conjugated is a secondary antibody which binds to dog IgG Fc part, in immunological assays.
CAS Number
9007-83-4
HS Code
38221900
Host
Goat
Reactivity
Dog IgG, Fc part
Not reactive in
It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion.
Immunogen
Purified dog IgG isolated from dog serum.
Clonality
Polyclonal
Applications
Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Field of Research
Anti-Dog
Dilution
1 : 100-250 (IHC and ICC parrafin sections), 1 : 500-5000 (ELISA), The optimum working dilution should be established by titration by end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Purity
Protein G purified goat IgG.
Format
Lyophilized from PBS pH 7.2
Reconstitution
For reconstitution add 1 ml of sterile destilled water
Precautions
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antibody has not been tested in detail.No preservative added, as it may interfere with the antibody activity.
Additionnal Information
The biotin/protein molar ratio is approximately 6.7.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stored in 4°C up to 7 days. Working dilutions should be stored at 4°C and not frozen. If any precipitate occurs, it should be removed by centrifugation.
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