Anti-Homogalacturonan-1 (clone CCRC-M38)
Product Specifications
Background
Homogalacturonan (HG) is a pectic polysaccharide, composed of α-1,4 linked galacturonic acid residues. Pectin consists of complex net of polysacchari.des which are found in the primary cell walls of terrestial plants and are enriched in non-woody parts. Pectin acts as an extracellular glue which binds cells together.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
MeBSA-conjugated Arabidopsis thaliana seed mucillage (Hemogalacturonan), non-covalent, Epitope structure for crabohydrate antigen: de-estrified homogalacturonan (DP>4)
Clonality
Monoclonal
Applications
ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Field of Research
Dr. Hahn collection, University of Georgia
Dilution
1:10 (IHC)
Purity
Cell culture supernatant.
Format
Liquid
Precautions
This antibody binds to a de-esterified ?-1,4 linked homogalcturonan epitope with a degree of polymerizaion of 4 or more, It does not bind to a homogalacturonan trimer
Additionnal Information
This antibody recognises fully de-esterified ?-1,4 linked homogalcturonan (HG) epitope with a degree of polymerization (DP) of four or higher (DP>4), Does not recognize a homogalacturonan trimer
References & Citations
Płachno et al. (2025) . The Localization of Cell Wall Components in the Whole-Mount Immunolabeled Nepenthes Digestive Glands.Int J Mol Sci. 2025 Sep 19;26 (18) :9174.doi: 10.3390/ijms26189174. Płachno at el. (2025) .Cell Wall Microdomains Analysis in the Quadrifids of Utricularia dichotoma. Int J Mol Sci. 2025 Jan 20;26 (2) :832. doi: 10.3390/ijms26020832. Pattathil et al. (2012) . Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6. Patathil et al. (2010) . A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153 (2) :514-25.doi: 10.1104/pp.109.151985.
Storage Conditions
Antibody can be stored up to 1 month at 4°C, and over 1 month at -80°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Homogalacturonan (HG) backbone-1 clade of antibodies and binds to a de-esterified ?-1,4 linked homogalcturonan (HG) epitope (DP>4)
Isotype
IgG1
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