What is the catalog number for Anti-AGO10 | Argonaute 10?

The catalog number for Anti-AGO10 | Argonaute 10 is AS15 3071.

What sizes are available for Anti-AGO10 | Argonaute 10?

Anti-AGO10 | Argonaute 10 is available in the following size(s): 50 µg.

Is Anti-AGO10 | Argonaute 10 in stock?

Anti-AGO10 | Argonaute 10 is currently made to order / not held in immediate stock. Contact us for lead time.

Anti-AGO10 | Argonaute 10

Product Specifications

Background

AGO10 is involved in miRNA binding, and in RNA-mediated posttranscriptional gene silencing (PTGS) .

CAS Number

9007-83-4

HS Code

38221900

Gene ID

834403

UniProt

Q9XGW1

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

Zea mays

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO10 protein sequence, Uniprot: Q9XGW1, TAIR: AT5G43810

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Plant RNA

Dilution

1 : 10 000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

110.9 kDa

Precautions

AGO expression may be cell/tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Seedlings can be used as a negative control.Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

References & Citations

Sun et al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7 (32) :eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Oliver & Martinez. (2021) Accumulation dynamics of ARGONAUTE proteins during meiosis in Arabidopsis. Plant Reprod. 2021 Nov 23. doi: 10.1007/s00497-021-00434-z. Epub ahead of print. PMID: 34812935.Sprunck et al. (2019) . Elucidating small RNA pathways in Arabidopsis thaliana egg cells.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

A. lyrata, B. napus, C. rubella, C. clementina, C. sinensis, E. salsugineum, G. arboreum, G. raimondii. M. truncatula, N. benthamiana, Poplar sp.Species of your interest not listed? Contact us