Goat anti-Mouse IgG2, FITC conjugated
Product Specifications
Background
Goat anti-mouse IgG2, FITC (fluorescein-5-isothiocyanate) conjugatedis a secondary antibody which is subclass specific and binds to mouse IgG1 in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody.
Host
Goat
Reactivity
Mouse IgG2a and IgG2b, subclass specific
Not reactive in
It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Immunogen
Purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b,
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)(frozen), Immunofluorescence (IF)
Dilution
1 : 20-1 :80 The optimum working dilution of each conjugate should be established by titration before being used, Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal
Purity
Immunogen affinity purified goat IgG.
Format
Lyophilized
Reconstitution
For reconstitution, add 1 ml of sterile distilled water
Storage Conditions
Stored lyophilize material at 4°C and for long time storage at -20°C. After reconstirution make aliquots and Store at -20°C to avoid repeated freeze-thaw cycles. Prior to use, thaw the aliquot slowly in the dark at ambient temperature, spin down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7,2). Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day.
Product Datasheet
https://cdn.gentaur.com/products/451/686bf4632da8eae897d65091/datasheet/as15 3052.pdf
CAS Number
9007-83-4
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