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Anti-RFP | Red Fluorescent Protein (clone RF5R)

Product Specifications

Background

RFP (Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide

Clonality

Monoclonal

Applications

Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

BFP/FITC/GFP/RFP/TRITC/YFP

Dilution

1 : 1000-3000 (WB)

Purity

Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.

Format

Liquid

Precautions

Antibody is purified on Protein A.Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.

Additionnal Information

Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally

References & Citations

Bizan et al. (2025) . Arabidopsis MYB47 and MYB95 transcription factors regulate jasmonate-inducible ER-body formation. Commun Biol . 2025 Sep 26;8 (1) :1377. doi: 10.1038/s42003-025-08863-6. Cecchini et al. (2020) . Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.

Storage Conditions

Store at -20°C for up to 2 years.

Isotype

IgG1

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