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Anti-RFP | Red Fluorescent Protein (clone RF5R)

Product Specifications

Background

RFP(Red Fluorescent Protein)is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection.

Host

Mouse

Reactivity

Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated RFP from theDiscosoma sp.(sea anemone) N-terminal peptide

Clonality

Monoclonal

Applications

Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)

Dilution

1 : 1000-3000 (WB)

Purity

Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide.

Format

Liquid

Precautions

Antibody is purified on Protein A.Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.

References & Citations

Cecchiniet al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031.

Storage Conditions

Store at -20°C for up to 2 years.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70a994c49e6dc66fa42/datasheet/as15 3028.pdf

CAS Number

9007-83-4

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