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Anti-V5 (clone V5, E10)

Product Specifications

Background

V5-tag is a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

V5-tagged fusion proteins

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide

Clonality

Monoclonal

Applications

Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

DIG/HA/HSV/KT3/Myc/S/T7/V5/VSV-G

Dilution

1 : 500-1 :2000 (IHC), 1 : 1000-1: 3000 (WB)

Purity

Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.

Format

Liquid

Reconstitution

For reconstitution add 50 µl of sterile water

Precautions

Antibody is purified on Protein A.For western blot incubate antibody for one hour at room temperature.

Additionnal Information

Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally

Storage Conditions

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://www.agrisera.com/en/artiklar/v5.pdf

Isotype

IgG1

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