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Anti-GFP | Green Fluorescence Protein, peroxidase conjugated

Product Specifications

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.

HS Code

38221900

UniProt

P42212

Host

Rabbit

Reactivity

Native GFP, Recombinant GFP (E, coli), all variants of GFP

Immunogen

Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)

Field of Research

BFP/FITC/GFP/RFP/TRITC/YFP

Dilution

1 : 5000-1 : 25 000 (ELISA), 1:50 (IG), 1: 100-1:250 (IHC), 1 : 2000-1 : 10 000 (WB)

Purity

Immunogen affinity purified serum in 10 mM Tris pH 8. Contains 0.02 % sodium azide. Soybean persoxidase conjugated.

Format

Liquid

Precautions

Minimal cross-reactivity withE.coliproteins

Additionnal Information

The antibody is detecting native YFP protein, recombinant protein from E.coli and native GFP-fusion proteins and all variants of GFP.

Storage Conditions

Store at 4°C or in small aliquotes at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://www.agrisera.com/en/artiklar/gfp-green-fluorescence-protein-peroxidase-conjugated-.pdf

CAS Number

9007-83-4

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