Anti-GFP | Green Fluorescence Protein, peroxidase conjugated
Product Specifications
Background
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.
CAS Number
9007-83-4
HS Code
38221900
UniProt
P42212
Host
Rabbit
Reactivity
Native GFP, Recombinant GFP (E, coli), all variants of GFP
Immunogen
Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Clonality
Polyclonal
Applications
ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)
Field of Research
BFP/FITC/GFP/RFP/TRITC/YFP
Dilution
1 : 5000-1 : 25 000 (ELISA), 1:50 (IG), 1: 100-1:250 (IHC), 1 : 2000-1 : 10 000 (WB)
Purity
Immunogen affinity purified serum in 10 mM Tris pH 8. Contains 0.02 % sodium azide. Soybean persoxidase conjugated.
Format
Liquid
Precautions
Minimal cross-reactivity withE.coliproteins
Additionnal Information
The antibody is detecting native YFP protein, recombinant protein from E.coli and native GFP-fusion proteins and all variants of GFP.
Storage Conditions
Store at 4°C or in small aliquotes at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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