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Anti-GFP | Green Fluorescence Protein (total IgY)

Product Specifications

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

CAS Number

9007-83-4

HS Code

38221900

UniProt

P42212

Host

Chicken

Reactivity

Native GFP, Recombinant GFP (E, coli), all variants of GFP

Immunogen

Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Clonality

Polyclonal

Applications

ELISA (ELISA), Western blot (WB)

Field of Research

BFP/FITC/GFP/RFP/TRITC/YFP

Dilution

1 : 5000-1 : 25 000 (ELISA), 1 : 2000-1 : 10 000 (WB)

Purity

Total IgY in 10 mM TRIS, 0,15 mM NaCl, pH 8. Contains 0.02 % sodium azide.

Format

Liquid

Precautions

Minimal cross-reactivity withE.coliproteins

Storage Conditions

Store at 4°C; make aliquots to avoid working with a stock. Or Store in small aliquots at -20°C, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

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