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Anti-GFP | Green Fluorescence Protein (affinity purified)

Product Specifications

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

CAS Number

9007-83-4

HS Code

38221900

UniProt

P42212

Host

Rabbit

Reactivity

Native GFP, Recombinant GFP (E, coli), all variants of GFP, including EGFP

Immunogen

Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212

Clonality

Polyclonal

Applications

ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)

Field of Research

BFP/FITC/GFP/RFP/TRITC/YFP

Dilution

1 : 5000-1 : 25 000 (ELISA), 1: 100 - 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Liquid

Precautions

Minimal cross-reactivity withE.coliproteins.Antibody reactivity to mVenus or EBFP2 has not yet been determined.

Additionnal Information

10 mM TRIS buffer pH 8,0 containing 0,02% sodium azide

References & Citations

Wang et al. (2025) . A receptor antagonist counterbalances multiple systemin phytocytokines in tomato. Cell . 2025 Aug 12:S0092-8674 (25) 00866-9. doi: 10.1016/j.cell.2025.07.044. Pinczés et al. (2024) . Viral coat proteins decrease the gene silencing activity of cognate and heterologous viral suppressors. Sci Rep. 2024 Dec 28;14 (1) :31008. doi: 10.1038/s41598-024-81998-4. Canal et al. (2024) .Cytochrome c levels affect the TOR pathway to regulate growth and metabolism under energy-deficient conditions. New Phytol. 2024 Mar;241 (5) :2039-2058. Sun et al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7 (32) :eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Stelate et al. (2021) Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. Biomolecules. 2021 Sep 26;11 (10) :1407. doi: 10.3390/biom11101407. PMID: 34680040; PMCID: PMC8533460.Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12 (10) :1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Kulich et al. (2018) . Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693.

Storage Conditions

Store at -20°C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

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