Anti-His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)
Product Specifications
Background
Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
6xHis tagged proteins
Immunogen
Polyhistidine (HHHHHH) tagged polypeptide
Clonality
Monoclonal
Applications
Western blot (WB)
Field of Research
BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...
Dilution
1: 5000 - 10 000 (WB)
Purity
Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Molecular Weight
Depends upon fusion partner
Precautions
Final primary antibody dilution, depend upon amount of Hisx6 tagged protein in analyzed sample.
Additionnal Information
Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4) Concentration: 0.1mg/ml
Storage Conditions
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.
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