Anti-His-tag | 6xHis, ALP Conjugated (Clone AD1,1,10)

Product Specifications

Background

Mouse anti-6x Histidine is a primary antibody which binds to 6x Histidine and is directly conjugated to ALP, Alkaline Phosphatase. This is of advantage to shorten assay time by no need to use a secondary antibody to 6x Histidine tag.

CAS Number

9007-83-4

HS Code

38221900

Host

Mouse

Reactivity

6xHis tagged proteins

Immunogen

Polyhistidine (HHHHHH) tagged polypeptide

Clonality

Monoclonal

Applications

Western blot (WB)

Field of Research

BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...

Dilution

1: 5000 - 10 000 (WB)

Purity

Immunogen affinity purified in 25 mM Tris, 150 mM NaCl, 5 mM MgCl2, 0.12 mM ZnCl2, pH 7.4. with 2 mM sodium azide.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

Depends upon fusion partner

Precautions

Final primary antibody dilution, depend upon amount of Hisx6 tagged protein in analyzed sample.

Additionnal Information

Antibody is provided in: 25 mM Tris, 150 mM Sodium Chloride, 5 mM Magnesium Chloride, 0.12 mM Zinc Chloride, 2 mM Sodium Azide (pH 7.4) Concentration: 0.1mg/ml

Storage Conditions

Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and Store at -20°C.