Anti-NAD6 | NADH-ubiquinone oxidoreductase chain 6

Product Specifications

Background

NAD6 (NADH-ubiquinone oxidoreductase chain 6)is involved in cellular respiration, oxidation-reduction process. Protein is a part of mitochondrial respiratory chain complex I and displays NADH dehydrogenase activity.

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjuagted peptide derived fromArabdiopsis thalianasequence, UniProt:G1C2Y0,TAIR:ATMG00270

Clonality

Polyclonal

Applications

Western blot (WB)

Dilution

1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

23.4 kDa (Arabidopsis thaliana), 25 kDa (Zea mays)

Precautions

There is a cross-reaction with a thylakoid protein, therefore this antibody may not be usable for western blots of total leaf extracts

References & Citations

Weiet al. (2019). Arabidopsis mtHSC70-1 plays important roles in the establishment of COX-dependent respiration and redox homeostasis. J Exp Bot. 2019 Aug 6. pii: erz357. doi: 10.1093/jxb/erz357.Colasdes Francs-Small et al. (2018). Targeted cleavage of nad6 mRNA induced by a modified pentatricopeptide repeat protein in plant mitochondria. Commun Biol. 2018 Oct 11;1:166. doi: 10.1038/s42003-018-0166-8.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.