Anti-FNR | Ferredoxin-NADP+-oxidoreductase

Product Specifications

Background

FNR (ferredoxin-NADP+-oxidoreductase) catalyzes reduction of NADP+ using Fd that has accepted electrons from photosystem II in the final step of linear photosynthetic electron transport. In higher plants FNRs are encoded by a small multiple gene family (two chloroplast-targeted FNR in Arabidopsis and rice and three isoenzymes in maize) . All forms are evenly distributed between the thylakoids and stroma.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

5722854

Host

Rabbit

Reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sorokiniana, Synechococcus sp. PCC 6803

Not reactive in

Oryza sativa, Spinacia oleracea, Zea mays

Immunogen

Purified, native FNR from Chlamydomonas reinhardtii, UniProt: A8J6Y8

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Electron transfer

Dilution

1 : 1000-1 : 3000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

35 kDa

Precautions

For detection inChlorella sorokiniana, SynechococcusPCC 6803 higher load per well needs to be applied.This antibody recognizes allArabidopsis thalianaFNR isoforms.This product can be sold with ProClin if requested

Additionnal Information

Arabidopsis has four FNR proteins, two of them are found in leaves (LFNR1 and LFNR2) while the other two in roots (RFNR1 and RFNR2) . Absence of one of leaf FNR results in a decrease in the amount of FNR while absence of both of them is lethal.

References & Citations

Tiwari et al. (2024) .Differential FeS cluster photodamage plays a critical role in regulating excess electron flow through photosystem I. Nat Plants. 2024 Oct;10 (10) :1592-1603. doi: 10.1038/s41477-024-01780-2. Liu et al. (2024) . Interactome Analysis of ClpX Reveals Its Regulatory Role in Metabolism and Photosynthesis in Cyanobacteria. J Proteome Res. 2024 Apr 5;23 (4) :1174-1187. doi: 10.1021/acs.jproteome.3c00610. Zhang et al. (2020) . Enhanced Relative Electron Transport Rate Contributes To Increased Photosynthetic Capacity In Autotetraploid Pak Choi. Plant Cell Physiol. 2020 Jan 6. pii: pcz238. doi: 10.1093/pcp/pcz238.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Catalpa bungei, Coccomyxa subellipsoidea (strain C-169), Gonium pectorale, Monoraphidium neglectum, Noccaea caerulescens, Ostreococcus lucimarinus (strain CCE9901), Volvox carteriSpecies of your interest not listed? Contact us