Anti-FNR | Ferredoxin-NADP+-oxidoreductase
Product Specifications
Background
FNR (ferredoxin-NADP+-oxidoreductase) catalyzes reduction of NADP+ using Fd that has accepted electrons from photosystem II in the final step of linear photosynthetic electron transport. In higher plants FNRs are encoded by a small multiple gene family (two chloroplast-targeted FNR in Arabidopsis and rice and three isoenzymes in maize) . All forms are evenly distributed between the thylakoids and stroma.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
5722854
Host
Rabbit
Reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sorokiniana, Synechococcus sp. PCC 6803
Not reactive in
Oryza sativa, Spinacia oleracea, Zea mays
Immunogen
Purified, native FNR from Chlamydomonas reinhardtii, UniProt: A8J6Y8
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Electron transfer
Dilution
1 : 1000-1 : 3000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
35 kDa
Precautions
For detection inChlorella sorokiniana, SynechococcusPCC 6803 higher load per well needs to be applied.This antibody recognizes allArabidopsis thalianaFNR isoforms.This product can be sold with ProClin if requested
Additionnal Information
Arabidopsis has four FNR proteins, two of them are found in leaves (LFNR1 and LFNR2) while the other two in roots (RFNR1 and RFNR2) . Absence of one of leaf FNR results in a decrease in the amount of FNR while absence of both of them is lethal.
References & Citations
Tiwari et al. (2024) .Differential FeS cluster photodamage plays a critical role in regulating excess electron flow through photosystem I. Nat Plants. 2024 Oct;10 (10) :1592-1603. doi: 10.1038/s41477-024-01780-2. Liu et al. (2024) . Interactome Analysis of ClpX Reveals Its Regulatory Role in Metabolism and Photosynthesis in Cyanobacteria. J Proteome Res. 2024 Apr 5;23 (4) :1174-1187. doi: 10.1021/acs.jproteome.3c00610. Zhang et al. (2020) . Enhanced Relative Electron Transport Rate Contributes To Increased Photosynthetic Capacity In Autotetraploid Pak Choi. Plant Cell Physiol. 2020 Jan 6. pii: pcz238. doi: 10.1093/pcp/pcz238.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Catalpa bungei, Coccomyxa subellipsoidea (strain C-169), Gonium pectorale, Monoraphidium neglectum, Noccaea caerulescens, Ostreococcus lucimarinus (strain CCE9901), Volvox carteriSpecies of your interest not listed? Contact us
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