Anti-DYKDDDDK (binds to Sigma FLAG®, clone FG4R)
Product Specifications
Background
FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
KLH-conjugated DYKDDDDK (FLAG) synthetic peptide
Clonality
Monoclonal
Applications
Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Field of Research
BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...
Dilution
1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature), 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user
Purity
Total IgG. Protein A purified in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
Format
Liquid
Additionnal Information
Working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.Antibody is purified on Protein A.
Storage Conditions
Store at -20°C for up to 2 years.
Isotype
IgG1
Documents
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