Anti-ATG4 | Autophagy protein 4 (algal)

Product Specifications

Background

ATG4 (Autophagy protein 4) is a protein with cyteine-type endopeptidase activity involved in autophagy process.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

819020

UniProt

Q8S929#Q8S929-2

Host

Rabbit

Reactivity

Chlamydomonas reinhardtii, Chlorococcum dorsiventrale

Not reactive in

Arabidopsis thaliana, Capsicum annuum, Nicotiana tabacum

Immunogen

Recombiant ATG4 of Chlamydomonas reinhardtii, Gene ID Cre12.g510100

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Autophagy-related and Ubiquitin-like Proteins

Dilution

1 : 5000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

64.2 | 70 kDa

Precautions

Technical details how to work with this antibody are provided here:Pérez-Pérezet al. (2016). Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172(4):2219-2234.

Additionnal Information

This antibody is recognizing 25 ng of recombinant CrATG4This product can be sold containing ProClin if requested.

References & Citations

Chen et al. (2016) . The role of nitric oxide signalling in response to salt stress in Chlamydomonas reinhardtii. Planta. 2016 Sep;244 (3) :651-69. doi: 10.1007/s00425-016-2528-0. Epub 2016 Apr 26.Pérez-Pérez et al. (2016) . Control of Autophagy in Chlamydomonas Is Mediated through Redox-Dependent Inactivation of the ATG4 Protease. Plant Physiol. 2016 Dec;172 (4) :2219-2234.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.