Anti-UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker)
Product Specifications
Background
UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011) . Alternative name: UTP--glucose-1-phosphate uridylyltransferase.
CAS Number
9007-83-4
HS Code
38221900
UniProt
Q43772
Host
Rabbit
Reactivity
Arabidopsis thaliana, Hordeum vulgare, Zea mays
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
His-tagged, full length Hordeum vulgare UGPase, overexpressed and purified from E.coli, UniProt: Q43772.1
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Carbohydrates
Dilution
1 : 10 000 (WB)
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
52 kDa
Precautions
This antibody is also recognizing recombinant UGPase, below 0,5 pmol
Additionnal Information
Cellular [compartment marker] of cytoplasm, UGPse is a cytoplasmic protein Martz et al, (2002)
References & Citations
Martín-Merchán et al. (2024) . Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387.Kleczkowski LA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press) .
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Bambusa oldhamii, Brassica pekinensis, Brassica rapa, Capsicum annuum, Cucumis sativus, Dendrobium catenatum, Dendrocalamus sinicus, Glycine max, Gossipium hirsutum, Lycopersicum esculentum, Lycopersicum chilense, Marchantia polymorpha, Oryza sativa, Picea glauca, Populus sp., Solanum tuberosum, Populus tremula, Ricinus communis, Saccharum officinarum, Vitis vinifera, for more species, please Species of your interest not listed? inquireSpecies of your interest not listed? Contact us
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