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Anti-TrxM1/M2 | Thioredoxin M1/M2 (chloroplastic)

Product Specifications

Background

TrxM1/M2 (Thioredoxin M1/M2, chloroplasticT)is a thiol-disulfide oxidoreductase involved in the redox regulation of enzymes of both reductive pentose phosphate pathway (Calvin-Benson cycle) and oxidative pentose phosphate pathway. Under reducing conditions, activates the glyceraldehyde-3-phosphate dehydrogenase and the phosphoribulokinase, and inhibits. the glucose-6-phosphate dehydrogenase. Activates NADP-malate dehydrogenase.

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

Marchantia polymorpha, Physcomitrella patens

Immunogen

KLH-conjugated peptide, derived fromArabidopsis thalianaTrxM1 UniProt:O48737, TAIR:AT1G03680and TrxM2 UniProt:Q9SEU8, TAIR:AT4G03520

Clonality

Polyclonal

Applications

Western blot (WB)

Dilution

1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

20 | 14 kDa

Precautions

5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.To work with this antibody chloroplast fraction has to be used.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70b994c49e6dc66ff62/datasheet/as14 2809.pdf

CAS Number

9007-83-4

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