Anti-NBR1 | Autophagy substrate NBR1 (affinity purified)
Product Specifications
Background
NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.
CAS Number
9007-83-4
HS Code
38221900
Gene ID
828571
UniProt
Q9SB64
Host
Rabbit
Reactivity
Arabidopsis thaliana
Not reactive in
Citrus sinensis, Nicotiana tabacum
Immunogen
UBA2 domain of NBR1 of Arabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690
Clonality
Polyclonal
Applications
Western blot (WB)
Field of Research
Pathogen attack
Dilution
1 : 500-1 : 5000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
75 | 100 kDa
Precautions
A specific extraction method and tissue type needs to be used as described in Minina et al, (2013), Dilution in western blot depends upon amount of NBR1 in the sample
References & Citations
Li et al. (2024) . Stress granules sequester autophagy proteins to facilitate plant recovery from heat stress. Nat Commun . 2024 Dec 30;15 (1) :10910. doi: 10.1038/s41467-024-55292-w.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Prediction Reactivity
Brassicaceae family, Solanum lycopersicumSpecies of your interest not listed? Contact us
Documents
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