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Anti-NBR1 | Autophagy substrate NBR1

Product Specifications

Background

NBR1 (Autophagy substrate NBR1)is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.

Host

Rabbit

Reactivity

Arabidopsis thaliana, Physcomitrium patens

Not reactive in

Citrus sinensis, Nicotiana tabacum

Immunogen

UBA2 domain of NBR1 ofArabidopsis thaliana, fused with GST, UniProt:Q9SB64, TAIR:AT4G24690

Clonality

Polyclonal

Applications

Immunolocalization (IL), Western blot (WB)

Dilution

1: 1000 (IL), 1 : 500-1 : 5000 (WB)

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

75 | 100 kDa

Precautions

Specific extraction method and tissue type needs to be used as described in Minina et al, (2013), Dilution in western blot depends upon amount of NBR1 in the sample

References & Citations

Yanet al. (2024). Autophagy modulates Arabidopsis male gametophyte fertility and controls actin organization. Nat Commun . 2024 Nov 21;15(1):10071. doi: 10.1038/s41467-024-54468-8.Mosessoet al. (2024). Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation. Nat Commun. 2024 Jun 19;15(1):5188. doi: 10.1038/s41467-024-49485-6.Lanet al. (2024).Clathrin Light Chains negatively regulate plant immunity by hijacking the autophagy pathway. Plant Commun. 2024 Apr 30:100937.doi: 10.1016/j.xplc.2024.100937.Zenget al. (2023) The plant unique ESCRT component FREE1 regulates autophagosome closure. Nat Commun. 2023 Mar 30;14(1):1768. doi: 10.1038/s41467-023-37185-6.Rodriguezet al. (2020). Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells. bioRxiv doi.org/10.1101/747410Calero-Mu�±ozet al. (2019). Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 Sep;42(9):2696-2714. doi: 10.1111/pce.13597.Jiaet al. (2019). Noncanonical ATG8-ABS3 interaction controls senescence in plants. Nat Plants. 2019 Feb;5(2):212-224. doi: 10.1038/s41477-018-0348-x.Hackenberget al. (2013). Catalase and NO CATALASE ACTIVITY1 promote autophagy-dependent cell death in Arabidopsis. Plant Cell. 2013 Nov;25(11):4616-26. doi: 10.1105/tpc.113.117192. Epub 2013 Nov 27.Mininaet al. (2013). Autophagy mediates caloric restriction-induced lifespan extension in Arabidopsis. Aging Cell. 2013 Apr;12(2):327-9. doi: 10.1111/acel.12048. Epub 2013 Feb 28. (method description in supplemental materials)Katsiarimpaet al. (2013). The Deubiquitinating Enzyme AMSH1 and the ESCRT-III Subunit VPS2.1 Are Required for Autophagic Degradation in Arabidopsis.Svenninget al. (2011). Plant NBR1 is a selective autophagy substrate and a functional hybrid of the mammalian autophagic adapters NBR1 and p62/SQSTM1. Autophagy. 2011 Sep;7(9):993-1010. Epub 2011 Sep 1. (original reference)

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70a994c49e6dc66f96f/datasheet/as14 2805.pdf

CAS Number

9007-83-4

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