Anti-NBR1 | Autophagy substrate NBR1

NBR1 (Autophagy substrate NBR1) is involved in selective autophagy process of damaged organelles, intracellular microbes, protein aggregates, cellular structures and specific soluble proteins. NBR1 mediates the process as an autophagic adapter. The protein has two UBA domains but only the C-terminal UBA domain bound ubiquitin. Alternative names: At4g24690, Putative uncharacterized protein F22K18.110.

Specific extraction method and tissue type needs to be used as described in Minina et al, (2013), Dilution in western blot depends upon amount of NBR1 in the sample

Guan et al. (2025) .Arabidopsis phospholipase Dζ2 facilitates vacuolar acidification and autophagy under phosphorus starvation by interacting with VATD. Cell Rep. 2025 Jul 15;44 (7) :116024. doi: 10.1016/j.celrep.2025.116024. Hoffman-Sommer et al. (2025) . The TRAPPC8/TRS85 subunit of the Arabidopsis TRAPPIII tethering complex regulates endoplasmic reticulum function and autophagy. Plant Physiol. 2025 Mar 1;197 (3) :kiaf042. doi: 10.1093/plphys/kiaf042. Julian et al. (2025) . ATG8ylation of vacuolar membrane protects plants against cell wall damage. Nat Plants. 2025 Feb;11 (2) :321-339.doi: 10.1038/s41477-025-01907-z. Yan et al. (2024) . Autophagy modulates Arabidopsis male gametophyte fertility and controls actin organization. Nat Commun . 2024 Nov 21;15 (1) :10071. doi: 10.1038/s41467-024-54468-8. Mosesso et al. (2024) . Arabidopsis CaLB1 undergoes phase separation with the ESCRT protein ALIX and modulates autophagosome maturation. Nat Commun. 2024 Jun 19;15 (1) :5188. doi: 10.1038/s41467-024-49485-6.Lan et al. (2024) .Clathrin Light Chains negatively regulate plant immunity by hijacking the autophagy pathway. Plant Commun. 2024 Apr 30:100937.doi: 10.1016/j.xplc.2024.100937. Rodriguez et al. (2020) . Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells. bioRxiv doi.org/10.1101/747410Calero-MuÃ?±oz et al. (2019) . Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves. Plant Cell Environ. 2019 Sep;42 (9) :2696-2714. doi: 10.1111/pce.13597.Jia et al. (2019) . Noncanonical ATG8-ABS3 interaction controls senescence in plants. Nat Plants. 2019 Feb;5 (2) :212-224. doi: 10.1038/s41477-018-0348-x.Hackenberg et al. (2013) . Catalase and NO CATALASE ACTIVITY1 promote autophagy-dependent cell death in Arabidopsis. Plant Cell. 2013 Nov;25 (11) :4616-26. doi: 10.1105/tpc.113.117192. Epub 2013 Nov 27.Minina et al. (2013) . Autophagy mediates caloric restriction-induced lifespan extension in Arabidopsis. Aging Cell. 2013 Apr;12 (2) :327-9. doi: 10.1111/acel.12048. Epub 2013 Feb 28. (method description in supplemental materials)

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.