Anti-Glyphosate

Glyphosate (N- (phosphonomethyl) glycine) is a herbicide of a broad action spectrum which is used to kill broadleaf weeds and grasses known to compete with commercial crops world-wide. Due to its physico-chemical properties glyphosate easily enters the aqueous environment where it is rapidly converted into its main metabolite AMPA (aminomethylphosphonic acid) .

This antibody was used to prepare anti-glyphosate immunoaffinity column which showed to be very specific and retained glyphosate but not AMPA or glufosinate. Recover of glyphosate was ca. 100 %.The antibody is applicable for any type of (waste) water sample. The detection limit is 2,5 μg/l, while the end concentration is 100 μg/l. The standard curve runs from 5 – 100 μg/l. For an optimal ELISA result it is crucial to use Greiner high affinity plates; As assay buffer 0,1M PBS, 1% BSA heat-treated, pH 7,4 is recommended.

Antibody is provided in 0.01M PBS, pH 7.2. Composition of PBS: 8 mM Na2HPO4; 2 mM KH2PO4, 137 mM NaCl; 2.68 mM KCl. pH 7.4For coating OVA-glyphosate or KLH-glyphosate can be used.

Majer-Baranyi et al. (2023) . Application of Highly Sensitive Immunosensor Based on Optical Waveguide Light-Mode Spectroscopy (OWLS) Technique for the Detection of the Herbicide Active Ingredient Glyphosate. Biosensors (Basel) . 2023 Jul 29;13 (8) :771. doi: 10.3390/bios13080771. Vestri et al. (2021) . LSPR immuno-sensing based on iso-Y nanopillars for highly sensitive and specific imidacloprid detection. J Mater Chem B. 2021 Nov 17;9 (44) :9153-9161. doi: 10.1039/d1tb01344k. PMID: 34694310.Viirlaid et al. (2019) . Immunoassay for rapid on-site detection of glyphosate herbicide. Environ Monit Assess. 2019 Jul 24;191 (8) :507. doi: 10.1007/s10661-019-7657-z.

Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing. For long term storage purposes in solution the addition of sodium azide at 0.02 % is advised with the appropriate precautions of use.