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Anti-Lhcb1-P | LHCII type I chlorophyll a/b-binding protein, phopshorylated

Product Specifications

Background

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

Arabidopsis thaliana, Echinochloa crus-galli, , Oryza sativa, Panicum miliaceum, Pisum sativum, Zea mays

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide RKT*VAKPKGP, where T* indicates phospho-Thr

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

LHC

Dilution

1 : 10 000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 25 µl of sterile water

Molecular Weight

25 | 25 kDa for Arabidopsis thaliana

References & Citations

Wójtowicz et al. (2025) . Shrink or expand? Just relax! Bidirectional grana structural dynamics as early light-induced regulator of photosynthesis. New Phytol . 2025 Jun;246 (6) :2580-2596. doi: 10.1111/nph.70175. Collombat et al. (2025) . Arabidopsis conditional photosynthesis mutants abc1k1 and var2 accumulate partially processed thylakoid preproteins and are defective in chloroplast biogenesis. Commun Biol . 2025 Jan 22;8 (1) :111. doi: 10.1038/s42003-025-07497-y.Nilsson et al. (2020) . PSB33 protein sustains Photosystem II in plant chloroplasts under UVA light. J Exp Bot. 2020 Sep 15; eraa427.doi: 10.1093/jxb/eraa427.Rudenko et al. (2019) . The role of carbonic anhydrase a-CA4 in the adaptive reactions of photosynthetic apparatus: the study with a-CA4 knockout plants. Protoplasma (2019) . https://doi.org/10.1007/s00709-019-01456-1Rantala and Tikkanen et al. (2018) . Phosphorylation-induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Rantala et al. (2017) . Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92 (5) :951-962. doi: 10.1111/tpj.13732.Fristedt et al. (2017) . PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Prediction Reactivity

Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Phaseolus vulgaris, Silene vulgaris, Solanum lycopersicum, Spinacia oleracea, Species of your interest not listed? Contact us

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