Anti-H+ATPase | Plasma membrane H+ATPase (chicken antibody)

The Plasma MembraneH+ATPaseis a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences includingArabidopsis thalianaATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 ofArabidopsis thalianaand hydrogen ATPase ofChlamydomonas reinhardtii(Q9FNS3)

VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.