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Anti-PsbA | D1 protein of PSII, phosphorylated

Product Specifications

Background

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.

Host

Rabbit

Reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Zea mays

Not reactive in

Cyanobacteria

Immunogen

KLH-conjugated synthetic peptide derived from available plant PsbA sequences with phosphorylated (T), includingArabidopsis thalianaUniProt:P83755, TAIR:AtCg00020,Oryza sativaP0C434and other higher plant PsbA sequences

Clonality

Polyclonal

Applications

Western blot (WB)

Dilution

1 : 10 000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

38 | 28-30 kDa

Precautions

This antibody is detecting phosphorylated PsbA protein.Antibodies are purified on a non-phosphorylated peptide.

References & Citations

Verhoeven and Kornkven(2023). Differences in photoprotective strategy during winter in Eastern white pine and white spruce. Tree Physiol. 2023 Oct 20:tpad131.doi: 10.1093/treephys/tpad131.Mazuret al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Upadhyayaand Jagadeeshwar Rao (2019). Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii. Plant Direct Volume 3, Issue 11.Xinget al. (2017). Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii. Front. Plant Sci., 15 December 2017. https://doi.org/10.3389/fpls.2017.02154.Liet al. (2015). Effect of hydrogen sulfide on D1 protein in wheat under drought stress. Acta Physiologiae Plantarum November 2015, 37:225.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Product Datasheet

https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6703e5/datasheet/as13 2669.pdf

CAS Number

9007-83-4

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