Anti-PsbA | D1 protein of PSII, phosphorylated
Product Specifications
Background
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.
Host
Rabbit
Reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Hordeum vulgare, Zea mays
Not reactive in
Cyanobacteria
Immunogen
KLH-conjugated synthetic peptide derived from available plant PsbA sequences with phosphorylated (T), includingArabidopsis thalianaUniProt:P83755, TAIR:AtCg00020,Oryza sativaP0C434and other higher plant PsbA sequences
Clonality
Polyclonal
Applications
Western blot (WB)
Dilution
1 : 10 000 (WB)
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Molecular Weight
38 | 28-30 kDa
Precautions
This antibody is detecting phosphorylated PsbA protein.Antibodies are purified on a non-phosphorylated peptide.
References & Citations
Verhoeven and Kornkven(2023). Differences in photoprotective strategy during winter in Eastern white pine and white spruce. Tree Physiol. 2023 Oct 20:tpad131.doi: 10.1093/treephys/tpad131.Mazuret al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Upadhyayaand Jagadeeshwar Rao (2019). Reciprocal regulation of photosynthesis and mitochondrial respiration by TOR kinase in Chlamydomonas reinhardtii. Plant Direct Volume 3, Issue 11.Xinget al. (2017). Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii. Front. Plant Sci., 15 December 2017. https://doi.org/10.3389/fpls.2017.02154.Liet al. (2015). Effect of hydrogen sulfide on D1 protein in wheat under drought stress. Acta Physiologiae Plantarum November 2015, 37:225.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
https://cdn.gentaur.com/products/451/6867f70b994c49e6dc6703e5/datasheet/as13 2669.pdf
CAS Number
9007-83-4
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