Donkey anti-Mouse IgG (H&L), DyLight® 350 conjugated

Donkey anti-Mouse IgG (H&L), DyLight®350 Conjugated is a secondary antibody conjugated to DyLight® 350, which binds to Mouse IgG (H&L) in immunological assays.DyLight® 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase Mouse IgG (H&L) DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on mouse IgG, light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins

Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.