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Goat anti-Biotin, DyLight® 350 conjugated

Product Specifications

Background

Goat anti-Biotin, DyLight® 350 Conjugated is a secondary antibody conjugated to DyLight® 350, which binds to Biotin in immunological assays.DyLight® 350 has Amax = 353 nm, Emax = 432 nm. Antibodies are purified using solid phase biotinDyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

CAS Number

9007-83-4

HS Code

38221900

Host

Goat

Immunogen

BSA-Biotin, KLH-Biotin

Clonality

Polyclonal

Field of Research

BC2/Biotin/BirA/DYKDDDDK/GST/Halo/His/...

Dilution

1 : 20-1 : 2000 for most applications

Purity

Immunogen affinity purified goat IgG.

Format

Lyophilized

Reconstitution

For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Additionnal Information

Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.This antibody reacts with BiotinThis antibody does not react with free BSA or free KLH.

Storage Conditions

Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

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