Anti-HA (rabbit polyclonal)
Product Specifications
Background
HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
CAS Number
9007-83-4
HS Code
38221900
Host
Rabbit
Reactivity
HA-tag
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Immunogen
KLH-conjugated synthetic peptide: YPYDVPDYA
Clonality
Polyclonal
Applications
Western blot (WB), immunoprecipitation (IP)
Field of Research
DIG/HA/HSV/KT3/Myc/S/T7/V5/VSV-G
Dilution
1 : 5000 (WB)
Purity
Affinity purified serum in PBS, pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Molecular Weight
Depends on the MW of the protein which is HA-tagged
Precautions
Important note: there is astrongcross reactivity between 25 -20 kDa inArabidopsis thalianawildtype.
References & Citations
Tanigawa et al. (2024) . FYVE1/FREE1 is involved in glutamine-responsive TORC1 activation in plants. iScience. 2024 Aug 26;27 (9) :110814. doi: 10.1016/j.isci.2024.110814. Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12 (4) :489-505. doi: 10.1016/j.molp.2019.01.002
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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