Anti-PIF5 | Phytochrome interacting factor 5 (rabbit antibody)

Product Specifications

Background

PIF5 (Phytochrome interacting factor 5) is a transcription factor which acts negatively in the phytochrome B signaling pathway and regulates PHYB at post-transcriptional level. Alternative protein names: AtbHLH65, bHLH65, bHLH065, Basic helix-loop-helix protein 65, PHYTOCHROME INTERACTING FACTORAS12 2112 3-LIKE 6, PIL6, Phytochrome interacting factor-like 6, Transcription factor EN 103.

CAS Number

9007-83-4

HS Code

38221900

Gene ID

825075

UniProt

Q84LH8

Host

Rabbit

Reactivity

Arabidopsis thaliana

Not reactive in

Marchantia polymorpha, Physcomitrella patens, Solanum lycopersicum, Vitis vinifera

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIF5, UniProt: Q84LH8, TAIR: AT3G59060

Clonality

Polyclonal

Applications

Western blot (WB)

Field of Research

Transcription regulation

Dilution

1 : 1000 (WB)

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Molecular Weight

49.3 | 62 kDa

Precautions

For detection of PIF5 please use the most sensitive detection reagent

Additionnal Information

PIF proteins are not that stable, therefore special precautions should be taken during extraction and whole procedure should be performed in as little light as possible (light green light) . Extraction of PIF proteins is described in Shen et al. (2007) .

References & Citations

Pham et al. (2018) . Dynamic regulation of PIF5 by COP1-SPA complex to optimize photomorphogenesis in Arabidopsis. Plant J. 2018 Aug 25. doi: 10.1111/tpj.14074.

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.